A simple method for the preparation of hepatocytes in good yield from obese rats is
described. Lipogenesis from [U-14C]- lactate, [U-14C]-glucose and tritiated water has been investigated in hepatocytes prepared from
both genetically obese ‘fatty’ rats and their lean littermates. Hepatocytes from obese
rats demonstrate elevated rates of fatty acid and sterol synthesis in contrast to
hepatocytes from lean animals. The rate of fatty acid synthesis is sensitive to the
dietary status of the animal prior to preparation. The enhanced lipogenesis in hepatocytes
from obese rate depends on lactate as a source of carbon rather than glucose. The
role of the liver in genetic obesity is discussed with particular reference to the
major precursor for hepatic lipogenesis.
Obese Rats - Hepatocytes - Lipogenesis - Glucose - Lactate
